primary human pulmonary artery endothelial cells (hpae)  (Lonza)

Journal: Cell reports

Article Title: FAK regulates tension transmission to the nucleus and endothelial transcriptome independent of kinase activity

doi: 10.1016/j.celrep.2024.114297

Figure Lengend Snippet:

Article Snippet: Primary Human Pulmonary Artery Endothelial Cells (HPAE) , Lonza Bioscience , Cat# CC-2530.

Techniques: Blocking Assay, Control, Recombinant, Activation Assay, SYBR Green Assay, Reverse Transcription, Plasmid Preparation, Transfection, Contraction Assay, Extraction, Activity Assay, Methylation, Software

Journal: Circulation research

Article Title: Activated Endothelial TGFβ1 Signaling Promotes Venous Thrombus Non-Resolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension

doi: 10.1161/CIRCRESAHA.119.315259

Figure Lengend Snippet: Representative images and quantitative analysis of the SMA-immunopositive area (A, C) and vascular channels lined by CD31-immunopositive cells (A, D) in 21-days-old venous thrombi of End.TGFβRII-WT (n=6 in C and n=7 in D) and End.TGFβRII-KO mice (n=6). Data shown in panel D represent the area lined by CD31-positive channels per total thrombus area (%). Scale bars represent 10 μm. Cells expressing endothelial and myofibroblast markers are highlighted by black arrows. Representative images of SMA- and CD31-immunopositive cells in vessel-rich regions of PEA tissue specimens from patients with CTEPH are shown in (B). Scale bars represent 100 μm. Representative flow cytometry dot blots after analysis of 21-days-old venous thrombi for the number of cells double-positive for CD31 and FSP1 (n=4 mice per group; E, F) or CDH5 and SMA (n=4 mice per group; G, H). Exact p-values, as determined by Mann-Whitney test, are shown in panels C, D, F and H.

Article Snippet: Isolation and cultivation: Human Umbilical Vein Endothelial Cells (HUVECs, PromoCell), Human Pulmonary Arterial Endothelial Cells (HPAECs, ATCC), Human Pulmonary Vein Endothelial Cells (HPVECs, CellBiologics) and endothelial cells outgrown from PEA tissue (CTEPH-ECs) were cultivated, as suggested by the supplier or as published.

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY

Journal: Circulation research

Article Title: Activated Endothelial TGFβ1 Signaling Promotes Venous Thrombus Non-Resolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension

doi: 10.1161/CIRCRESAHA.119.315259

Figure Lengend Snippet: Representative pictures after immunofluorescence confocal microscopy for VE-cadherin (CDH5) and SMA in CTEPH tissue specimens, endothelial cells outgrown from CTEPH tissue (CTEPH-ECs) and HPAECs (A). Representative confocal pictures for CDH5 and SMA in HPAECs and HUVECs with and without TGFβ1 stimulation (10 ng/ml for 7 days) to induce EndMT (B). Quantitative analysis of CDH5 (n=5 biological replicates; C), SMA (ACTA2; n=4 for CTEPH and n=6 for all others; D), SNAIL (n=3; E), ZEB1 (n=3; F), TWIST (n=5; G) and FGFR1 (n=4 for CTEPH and n=5 for all others; H) mRNA expression in HPAECs with and without TGFβ1 stimulation (10 ng/mL for 7 days) and CTEPH-ECs. Scale bars represent 10 μm. Exact p-values, as determined by One-Way ANOVA followed by Bonferroni’s multiple comparisons test (3 comparisons) and by Mann-Whitney test for the comparison of HPAECs with and without TGFβ1 stimulation, are shown in panels C-H. Non-significant p-values are not shown.

Article Snippet: Isolation and cultivation: Human Umbilical Vein Endothelial Cells (HUVECs, PromoCell), Human Pulmonary Arterial Endothelial Cells (HPAECs, ATCC), Human Pulmonary Vein Endothelial Cells (HPVECs, CellBiologics) and endothelial cells outgrown from PEA tissue (CTEPH-ECs) were cultivated, as suggested by the supplier or as published.

Techniques: Immunofluorescence, Confocal Microscopy, Expressing, MANN-WHITNEY, Comparison